Essay about Dna And Dna Into Pet 41a ( + )

3655 Words Jul 8th, 2015 15 Pages
The Process of Ligation, Transformation, and Isolation of EGFP cDNA Into pET-41a(+) By Using Miniprep, Restriction Digest, PCR, and Bioinformatics To Ultimately Create Recombinant Expression Plasmids.
INTRODUCTION:
Scientists in this day and age are now able to change DNA and create recombinant DNA, which is a huge indication of great possibilities for the future of genetics. The goal of these lab experiments was to be able to create recombinant DNA with the insert of egfp gene. EGFP also known as Enhanced Green Fluorescent Protein was originally isolated from the jellyfish Aequorea Victoria and was introduced into the scientific field as a tool to signal gene expression. When EGFP is exposed to Ultraviolet Light, the protein glows a very bright green color. The egfp gene extracted from the jellyfish Aequorea Victoria was then modified to be able to be used in a lab setting in order to indicate gene expression and help aid in protein targeting. In this lab experiment, the egfp gene was ligated into pET-41a(+) expression plasmid to see whether the plasmid was recombinant or non-recombinant. If the egfp gene is successfully ligated into the pET-41a(+) vector, the result will be a recombinant DNA plasmid. In these series of lab experiments, egfp gene was ligated into pET-41a(+) expression plasmid by cutting the plasmid with Not1 and Nco1 restriction enzymes. From the recombinant expression plasmid that had been made, the E. coli bacteria was transformed with recombinant…

Related Documents