The Spectrophotometer Analysis Of Escherichia Coli Essay

1111 Words Jul 21st, 2015 5 Pages
The spectrophotometer analysis of Escherichia coli growth during heat application

The purpose of this experiment is to determine the rate of Escherichia coli (E. coli) growth over 100 minutes in a liquid growth medium (control), and to determine the rate of E. coli growth over 100 minutes in a heated liquid growth medium. The prediction is that the control E. coli culture will grow at a faster rate exponentially over 100 minutes than the experimental bacteria culture. Consequently due to heat application, the experimental bacteria will not grow over the 100 minutes, and will either move into G0 of its cell cycle or will produce descending results as cell death occurs.
The null hypothesis for this experiment is that the experimental E. coli will not grow when heat is applied, unlike the control E. coli that will grow exponentially without an added environmental agent. The alternative hypothesis for this experiment is that the experimental E. coli and the control E. coli will have an exponential growth rate.

Methods
To prepare the control and experimental formulas, 20 mL of LB growth media and 20mL of E. coli are added to two separate test tubes. The control tube is placed into a rotating incubator, and the experimental tube is placed into a hot water bath between 70-80 degrees Celsius. To maintain this temperature, add hot water frequently to the bath. Next, add 2 mL of the control formula into a cuvette, and place into a spectrophotometer (after it has been zeroed at a…

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