The Use Of Luminol Fluorescence And Chemiluminescence By Acting As A Catalyst With Hydrogen Peroxide

920 Words Jul 19th, 2015 4 Pages
Recent research shows that AgNPs enhance luminol fluorescence and chemiluminescence by acting as a catalyst with hydrogen peroxide. When AgNPs interact with hydrogen peroxide they cause the peroxide to degrade into superoxide and hydroxyl radical, which also react with luminol to form 3-aminophthalate (Equations 2-5) [13]. The further degradation of hydrogen peroxide into other reactive oxygen species promotes higher fluorescence intensity. Figure 5 shows the increased fluorescence intensity of luminol, AgNPs and hydrogen peroxide in solution. However, when strontium is present in solution, the fluorescence intensity decreased to the same intensity promoted by hydrogen peroxide when AgNPs are not present. The strontium not only causes aggregation of the AgNP but also extensively reduces and stops the catalytic process in degradation of hydrogen peroxide. Since the amplification of luminol fluorescence takes place when present with AgNPs, comparing varying concentrations of AgNPs or results involving other silver forms to each other is not able to provide reliable results of ROS production. Therefore, only similar solutions compositions with the same silver species and concentration may compare when using luminol for detecting ROS production.

Figure 6 A-C shows the fluoresecence results for the production of reactive oxygen species (hydrogen peroxide, superoxide radical and hydroxide ion) promoted by interactions of silver forms within the leaves of P. stratiotes.…

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